Part:BBa_K5038010
LIRA_H142_11/12
Usage and Biology
LIRA_H142_11/12 is a LIRA system which could specifically detect hsa-miR-142-3p, and has the same detection principle to LIRA_H01. There is a complementary sequence of hsa-miR-142-3p on the loop of the hairpin structure, and the RBS and initiation codon AUG will be exposed when the complementary sequence is bound to the target RNA, thereby activates translation.
"11/12" indicates that this LIRA system has a stem-loop ratio of 11/12 when bound to the corresponding input, i.e., in the input sequence, 11 bases are bound to the stem of the LIRA hairpin and 12 bases are bound to the loop.
(Figure 1. Schematic of LIRA_H142_11/12)
Blue: miRNA binding site, miR-142-3p binds to this area
Purple: RBS
Yellow: AUG, initiation codon
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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